Actalent Manufacturing Associate, Pipeline Products Support in San Diego, California
Position Summary The pipeline product manufacturing associate will be responsible for supporting internal manufacturing operations by working as a cleanroom operator and verifier in the aseptic production of several biomolecules including but not limited to plasmid DNA, mRNA, guide RNA, proteins and small biomolecules Responsibilities These may include but are not limited to: • Production of plasmid DNA at small to medium scale: The associate will perform bacterial transformation, strain selection, small to medium scale bacterial culture, harvest, DNA isolation. • Production of mRNA at small to medium scale: The associate will generate DNA template, in vitro transcribe mRNA, purify and concentrate mRNA. • Production of small biomolecules and proteins: Aseptically reconstitute premade vials of certain biomolecules or proteins to the requested specifications for downstream use. • Perform certain in-process tests during the production campaigns to ensure that intermediate production products are of high quality • Apply their molecular biology knowledge to troubleshoot problems and improve production capabilities. • Maintain cleanroom standards, practices, and housekeeping according to standard operating procedures • Read and follow Standard Operating Procedures (SOPs) and manufacturing work instructions. Fill out batch records, file deviation reports etc. during the manufacturing process • Write or revise batch records, SOPs etc. • Be involved in the design, installation, and validation of new equipment and additional clean rooms • Coordinate equipment maintenance and instrument calibration
bacterial transformation, DNA isolation, Bacterial cell culture, in-process testing, MNR, DNA
Top Skills Details:
bacterial transformation,DNA isolation,Bacterial cell culture,in-process testing,MNR,DNA
Additional Skills & Qualifications:
Requirements, Knowledge, Skills and Abilities • Minimum of Bachelor’s degree in a chemical/biochemical engineering related discipline and at least 2 years of relevant work experience, ideally in a cleanroom environment manufacturing nucleic acids (an equivalent combination of education and experience may be considered) The ideal candidate will possess: • A good understanding of current Good Manufacturing and Documentation Practices • A strong understanding of molecular biology principles especially as they apply to mRNA/DNA production • Excellent aseptic technique • Experience working with commercially available kits that are used to produce research grade DNA and mRNA (pilot or commercial-scale nucleic acid manufacturing experience is a bonus) • Tissue culture experience (bonus) • The ability to communicate clearly and precisely, both orally and in writing • High attention to detail • Proficiency with the MS-Office suite of productivity software and basic computer skills • Ability to solve basic math problems such as those required in the execution of a molecular biology protocol • Ability to work on multiple tasks simultaneously and meet project deadlines • Experience working on cross-functional teams and a commitment to working in a fast-paced environment.
Actalent connects passion with purpose. Our scalable talent solutions and services capabilities drive value and results and provide the expertise to help our customers achieve more. Every day, our experts around the globe are making an impact. We’re supporting critical initiatives in engineering and sciences that advance how companies serve the world. Actalent promotes consultant care and engagement through experiences that enable continuous development. Our people are the difference. Actalent is an operating company of Allegis Group, the global leader in talent solutions.
The company is an equal opportunity employer and will consider all applications without regards to race, sex, age, color, religion, national origin, veteran status, disability, sexual orientation, gender identity, genetic information or any characteristic protected by law.