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Case Western Reserve University Research Assistant 2 in Cleveland, Ohio

POSITION OBJECTIVE

Working under general supervision, the Research Assistant 2 will plan and carry out research project in accordance with general plans. The goal of the project will be to culture stem cells and learn their potency and efficacy using in vitro tissue culture and in vivo modeling. This position will work with animals, as well as tissue obtained from humans.

 

ESSENTIAL FUNCTIONS 

  1. Plan and carry out project in accordance with general plans. Assist in the in vivo animal studies, using murine models of lung infection and inflammation. Perform flow cytometry and immunofluorescence to follow cells. Perform sterile techniques and extensive tissue culture of mesenchymal stem cells and transformed cell lines as well as primaries. (40%)
  2. Conduct a series of experiments to supply scientific information as part of a larger project. Utilizing a variety of bacteria, perform microbiology studies, culturing bacteria, sterile technique, counting colonies and managing a variety of growth mediums. Perform ELISA and Luminex based assays to follow biomarkers. Maintain meticulous documentation from experiments and collection of data. (20%)
  3. Collect and analyze data and present results in laboratory meetings. Specifically, bacterial counts, protein concentration, cytokine measurements, cell counts, and gene expression outcomes. These will be discussed in the laboratory meeting setting with the group for a team approach to pushing the studies together. (10%)
  4. May assist in developing improved techniques, projection methods or procedures. Work with both pre-established guidelines and assist in the development of new technologies or improved approaches to perform the functions of the job. Since the laboratory focuses on bench to bedside research, new techniques, reagents or approaches maybe incorporated to improve the sensitivity and specificity of the results. Quantifying not just cell counts, but their activity and response to new potential treatments. 10%)

  5. Oversee supply inventory and ordering. Maintain certain equipment facilities. (10%) 

     

NonEssential Functions

  1. Act as mentor to students, fellows and other new individuals in the laboratory. Train new staff and direct the work of students. (5%)
  2. Assist in manuscript or report preparation. (2.5%) 

  3. Perform other duties as assigned. (2.5%)

     

CONTACTS 

Department: Daily contact with supervisor and laboratory manager to discuss research and maintain workflow.

University: Occasional contact with other departments, investigators and technical staff to share information.

External: Limited contact with vendors or collaborators with the laboratory to exchange information.

Students: Frequent contact with student employees and student scholars to help with training and to exchange information.

 

SUPERVISORY RESPONSIBILITY

This position has no direct supervision of staff employees. Will be involved with students from high school through fellowship trainees. Train new staff; direct the work of students. 

 

QUALIFICATIONS

Education/Experience: Bachelor's degree and 1 to 3 years of related experience or Associate's degree in an approved biotechnology program and 2 to 4 years of experience required. 

 

REQUIRED SKILLS 1. Has knowledge of commonly used concepts, practices, and procedures within an immunology/microbiology laboratory as well as some appreciation for stem cell cultures. 2. Ability to operate laboratory equipment. 3. Must demonstrate compliance with Case Western Reserve University animal research and care (ARC) policies and procedures and compliance to regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines and other applicable regulatory guidelines. 4. Must demonst

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