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Actalent Cell Culture Technician in Berkeley, California

Cell Culture/Manufacturing Technician

Great opportunity to work with one of the TOP pharmaceutical companies in the world!

Overview:

You will be responsible for Cell Culture bioreactor system assembly and use, including control of gas supply to bioreactor, Micro-sparger assembly, Anti-Foam Emulsion system. Ability to troubleshoot and control processes.

  • Continuous Cells Separation equipment preparation and process/controls.

  • Membrane Absorption Chromatography process (e.g. Membrane Absorption process, Buffer and NaOH supply, cTCF supply, Capsule preparation, Inline dilution and Temperature controls).

  • Basic knowledge of protein chemistry and purification systems such as anion, cation, and immunoaffinity chromatography.

  • Membrane Absorber technology, viral removal techniques and tangential flow filtration technology. Large scale chromatography, TFF technology.

  • Fam iliarity with cGMP’s and applicable agency regulations

    Prepares process equipment (including but not limited to Bioreactors & ancillary equipment, Stainless Steel tanks, Pressure Transmitters, Flow Meters, Aggregate traps, Harvest discards, Transfer lines (buffers, cTCF), Hoses and small parts.

  • Cleans and Sterilizes equipment using appropriate CIP (Clean-In-Place), SIP (Steam-In Place) procedures.

  • Performs daily or routine process operations in the cell culture and purification of biotech and biological products (Kovaltry, Kogenate, and Jivi). Operates equipment for these processes including:

  • Inoculates and monitors bioreactors.

  • Processes cTCF material to Continuous Cell Separation (CCS).

  • Operates Chromatography, MAEF, MAVF and TFF skids.

  • Processes MAEF material using closed thaw process, step elution chromatography, Membrane Absorber and filter clearance technology, and gradient controlled SP column separation techniques.

  • Operates 50L, 200L and 1000L collection bag technology to ensure robust collection, mixing and dilution of products.

  • Performs PEGylation of JIVI in process material using TFF technology.

  • Operates automated bagging systems.

  • Blast Freezes product for storage.

  • o Dispenses chemicals and formulate solutions.

  • Prepares acids and bases as necessary.

  • Performs aseptic operations.

  • Takes, analyzes and submits samples as required throughout the process.

  • Monitors and controls operation with computer control systems.

  • Completes all routine check sheets.

  • Cleans equipment and maintains clean and orderly production facilities.

  • Performs in-depth troubleshooting and root cause analysis of Kovaltry, Kogenate, and Jivi equipment and process failures.

  • Contributes to continuous improvement opportunities.

  • Performs all operations in compliance with cGMP.

  • Performs other related duties as assigned.

    Required Qualifications:

    • Bachelor's Degree in scientific discipline

    • Relevant bio-tech industry experience in some or all of the following is preferred, and may be considered in lieu of some or all of the educational requirements above

  • Experience with bioreactor operation

  • Cell culture experience

    About Actalent

Actalent is a global leader in engineering and sciences services and talent solutions. We help visionary companies advance their engineering and science initiatives through access to specialized experts who drive scale, innovation and speed to market. With a network of almost 30,000 consultants and more than 4,500 clients across the U.S., Canada, Asia and Europe, Actalent serves many of the Fortune 500.

Diversity, Equity & Inclusion

At Actalent, diversity and inclusion are a bridge towards the equity and success of our people. DE&I are embedded into our culture through:

  • Hiring diverse talent

  • Maintaining an inclusive environment through persistent self-reflection

  • Building a culture of care, engagement, and recognition with clear outcomes

  • Ensuring growth opportunities for our people

The company is an equal opportunity employer and will consider all applications without regard to race, sex, age, color, religion, national origin, veteran status, disability, sexual orientation, gender identity, genetic information or any characteristic protected by law.

If you would like to request a reasonable accommodation, such as the modification or adjustment of the job application process or interviewing process due to a disability, please email actalentaccommodation@actalentservices.com (%20actalentaccommodation@actalentservices.com) for other accommodation options.

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